Molecular Pathology and Genetics of Low Grade Glioma, Focus on Diffuse Astrocytoma (WHO Grade II)

In a collaborative initiative between St. Jude Children’s Research Hospital and University of London, this project led by principal investigator Dr. David Ellison is a comprehensive study of grade II LGGs with a view to deriving a molecular diagnostic/stratification system and identifying novel markers for the development of targeted therapies. This strategy involves:

• Assessment and analysis of DNA/RNA from grade II LGGs for genome-wide CNAAs, subgroup gene expression/miRNA profiles, aberrant DNA methylation, signaling pathway analysis and specific candidate gene sequencing
• Search for mutations by paired end/cDNA sequencing
• Validation of molecular abnormalities on further FFPE samples and comparison with other LGGs and glioneuronal tumors

This project is a one-year study to provide an opportunity for both St. Jude and University of London to assess the potential of further collaboration to advance scientific understanding of LGG, particularly WSO II diffuse astrocytoma.

Funded in 2012.